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J Biotechnol. 2011 Sep 20;155(3):284-6. doi: 10.1016/j.jbiotec.2011.07.010. Epub 2011 Jul 28.

IC-tagged proteins are able to interact with each other and perform complex reactions when integrated into muNS-derived inclusions.

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Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia y Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), Universidad de Santiago de Compostela, Campus Vida S/N, 15782-Santiago de Compostela (A Coruña), Spain.


We have recently developed a versatile tagging system (IC-tagging) that causes relocation of the tagged proteins to ARV muNS-derived intracellular globular inclusions. In the present study we demonstrate (i) that the IC-tag can be successfully fused either to the amino or carboxyl terminus of the protein to be tagged and (ii) that IC-tagged proteins are able to interact between them and perform complex reactions that require such interactions while integrated into muNS inclusions, increasing the versatility of the IC-tagging system. Also, our studies with the DsRed protein add some light on the structure/function relationship of the evolution of DsRed chromophore.

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