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Int J Radiat Biol. 2011 Aug;87(8):791-801. doi: 10.3109/09553002.2011.568574.

Gene expression signatures of radiation exposure in peripheral white blood cells of smokers and non-smokers.

Author information

1
Center for Radiological Research, Columbia University Medical Center, New York, NY 10032, USA.

Abstract

PURPOSE:

The issue of potential confounding factors is critical to the development of any approach to radiation biodosimetry, and has not been fully addressed for gene expression-based approaches.

MATERIALS AND METHODS:

As a step in this direction, we have investigated the effect of smoking on the global radiation gene expression response in ex vivo-irradiated peripheral blood cells using microarray analysis. We also evaluated the ability of gene expression signatures to predict the radiation exposure level of ex vivo-exposed samples from smokers and non-smokers of both genders.

RESULTS:

We identified eight genes with a radiation response that was significantly affected by smoking status, and confirmed an effect of smoking on the radiation response of the four and a half LIM domains 2 (FHL2) gene using quantitative real-time polymerase chain reaction. The performance of our previously defined 74-gene signature in predicting the radiation dose to samples in this study was unaffected by differences in gender or smoking status, however, giving 98% correct prediction of dose category. This is the same accuracy as that found in the original study from which the signature was derived, using different donors.

CONCLUSION:

The results support the development of peripheral blood gene expression as a viable strategy for radiation biodosimetry.

PMID:
21801107
PMCID:
PMC3580190
DOI:
10.3109/09553002.2011.568574
[Indexed for MEDLINE]
Free PMC Article
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