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World J Gastroenterol. 2011 Jul 7;17(25):2992-3001. doi: 10.3748/wjg.v17.i25.2992.

Down-regulation of STAT3 expression by vector-based small interfering RNA inhibits pancreatic cancer growth.

Author information

1
Department of General Surgery, Affiliated First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China.

Abstract

AIM:

To evaluate the effect of RNA interference (RNAi) mediated silence of signal transduction and activation of transcription (STAT)3 on the growth of human pancreatic cancer cells both in vitro and in vivo.

METHODS:

STAT3 specific shRNA was used to silence the expression of STAT3 in pancreatic cancer cell line SW1990. The anti-growth effects of RNAi against STAT3 were studied in vitro and in experimental cancer xenografts in nude mice. The potential pathways involved in STAT3 signaling were detected using reverse transcription polymerase chain reaction and western blotting.

RESULTS:

The expression of the STAT3 was inhibited using RNAi in SW1990 cells. RNAi against STAT3 inhibited cell proliferation, induced cell apoptosis and significantly reduced the levels of CyclinD1 and Bcl-xL when compared with parental and control vector-transfected cells. In vivo experiments showed that RNAi against STAT3 inhibited the tumorigenicity of SW1990 cells and significantly suppressed tumor growth when it was directly injected into tumors.

CONCLUSION:

STAT3 signaling pathway plays an important role in the progression of pancreatic cancer, and silence of STAT3 gene using RNAi technique may be a novel therapeutic option for treatment of pancreatic cancer.

KEYWORDS:

Growth; Pancreatic cancer; RNA interference; Signal transduction and activation of transcription 3

PMID:
21799645
PMCID:
PMC3132250
DOI:
10.3748/wjg.v17.i25.2992
[Indexed for MEDLINE]
Free PMC Article

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