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PLoS One. 2011;6(7):e22088. doi: 10.1371/journal.pone.0022088. Epub 2011 Jul 15.

CBP/p300 and SIRT1 are involved in transcriptional regulation of S-phase specific histone genes.

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1
Institute of Molecular and Cell Biology, Singapore, Singapore.

Abstract

BACKGROUND:

Histones constitute a type of essential nuclear proteins important for chromatin structure and functions. The expression of major histones is strictly confined to the S phase of a cell cycle and tightly coupled to DNA replication.

METHODOLOGY/PRINCIPAL FINDINGS:

With RT-qPCR and ChIP assays, we investigated transcriptional regulation of the S-phase specific histone genes and found that the acetylation level of histones on core histone gene promoters fluctuated during cell cycle in a pattern similar to RNA polymerase II association. Further, we showed that CBP/p300 and SIRT1 were recruited to histone gene promoters in an NPAT-dependent manner, knockdown of which affected histone acetylation on histone gene promoters and histone gene transcription.

SIGNIFICANCE:

These observations contribute to further understanding of the mechanism by which the expression of canonical histone genes is regulated, and also implicate a link between histone expression and DNA damage repair and cell metabolism.

PMID:
21789216
PMCID:
PMC3137613
DOI:
10.1371/journal.pone.0022088
[Indexed for MEDLINE]
Free PMC Article
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