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Antimicrob Agents Chemother. 2011 Oct;55(10):4922-5. doi: 10.1128/AAC.00704-11. Epub 2011 Jul 25.

Extended-spectrum AmpC cephalosporinase in Acinetobacter baumannii: ADC-56 confers resistance to cefepime.

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1
Division of Infectious Diseases, University of Pittsburgh School of Medicine, Scaife Hall S829, 3550 Terrace Street, Pittsburgh, PA 15261, USA.

Abstract

ADC-56, a novel extended-spectrum AmpC (ESAC) β-lactamase, was identified in an Acinetobacter baumannii clinical isolate. ADC-56 possessed an R148Q change compared with its putative progenitor, ADC-30, which enabled it to hydrolyze cefepime. Molecular modeling suggested that R148 interacted with Q267, E272, and I291 through a hydrogen bond network which constrained the H-10 helix. This permitted cefepime to undergo conformational changes in the active site, with the carboxyl interacting with R340, likely allowing for better binding and turnover.

PMID:
21788456
PMCID:
PMC3186995
DOI:
10.1128/AAC.00704-11
[Indexed for MEDLINE]
Free PMC Article
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