Format

Send to

Choose Destination
See comment in PubMed Commons below
J Urol. 2011 Sep;186(3):1121-7. doi: 10.1016/j.juro.2011.04.107. Epub 2011 Jul 23.

The mechanoreceptor TRPV4 is localized in adherence junctions of the human bladder urothelium: a morphological study.

Author information

1
Department of Urology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands. d.janssen@uro.umcn.nl

Abstract

PURPOSE:

TRPV4 (transient receptor potential vanilloid 4 channel) is a nonselective cation channel involved in different sensory functions that was recently implicated in bladder mechanosensation. We investigated the cellular site of TRPV4 in bladder urothelium and explored a molecular connection between TRPV4 and urothelial adherence junctions.

MATERIALS AND METHODS:

We obtained healthy tissues sections from cystectomy in humans due to cancer in 3 and noncancerous conditions in 2. Besides human biopsies tissues from 7 normal and 7 TRPV4-/-mice, and the urothelial cell line RT4 were also used. Experiments were done with polyclonal antibody against TRPV4 (against the N-terminus of rat TRPV4). A molecular connection between TRPV4 and different adherence junction components was investigated using immunofluorescence, Western blot and immunoprecipitation.

RESULTS:

Results revealed TRPV4 on urothelial cell membranes near adherence junctions. Results were comparable in the urothelial cell line, human bladders and mouse bladders. Subsequent immunoprecipitation experiments established a molecular connection of TRPV4 to α-catenin, an integral part of the adherence junction that catenates E-cadherin to the actin-microfilament network.

CONCLUSIONS:

Results provide evidence for the location of TRPV4 in human bladder urothelium. TRPV4 is molecularly connected to adherence junctions on the urothelial cell membrane. TRPV4 coupling to a rigid intracellular and intercellular structural network would agree with the hypothesis that TRPV4 can be activated by bladder stretch.

PMID:
21784462
DOI:
10.1016/j.juro.2011.04.107
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center