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Environ Toxicol Pharmacol. 2009 Jul;28(1):37-41. doi: 10.1016/j.etap.2009.02.001. Epub 2009 Feb 11.

Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests.

Author information

1
Facultad de Agronomía y Veterinaria (FAV), Universidad Nacional de Río Cuarto (UNRC), Ruta 36, KM 601, 5800, Río Cuarto, Argentina; CONICET, Argentina.

Abstract

It was evaluated the genotoxicity of glyphosate which up to now has heterogeneous results. The comet assay was performed in Hep-2 cells. The level of DNA damage in the control group (5.42±1.83 arbitrary units) for tail moment (TM) measurements has shown a significant increase (p<0.01) with glyphosate at a range concentration from 3.00 to 7.50mM. In the chromosome aberrations (CA) test in human lymphocytes the herbicide (0.20-6.00mM) showed no significant effects in comparison with the control group. In vivo, the micronucleus test (MNT) was evaluated in mice at three doses rendering statistical significant increases at 400mg/kg (13.0±3.08 micronucleated erythrocytes/1000 cells, p<0.01). In the present study glyphosate was genotoxic in the comet assay in Hep-2 cells and in the MNT test at 400mg/kg in mice. Thiobarbituric acid reactive substances (TBARs) levels, superoxide dismutase (SOD) and catalase (CAT) activities were quantified in their organs. The results showed an increase in these enzyme activities.

PMID:
21783980
DOI:
10.1016/j.etap.2009.02.001

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