Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2011;6(7):e22225. doi: 10.1371/journal.pone.0022225. Epub 2011 Jul 14.

Assessment of α-synuclein secretion in mouse and human brain parenchyma.

Author information

1
Division of Basic Neurosciences, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.

Abstract

Genetic, biochemical, and animal model studies strongly suggest a central role for α-synuclein in the pathogenesis of Parkinson's disease. α-synuclein lacks a signal peptide sequence and has thus been considered a cytosolic protein. Recent data has suggested that the protein may be released from cells via a non-classical secretory pathway and may therefore exert paracrine effects in the extracellular environment. However, proof that α-synuclein is actually secreted into the brain extracellular space in vivo has not been obtained. We developed a novel highly sensitive ELISA in conjugation with an in vivo microdialysis technique to measure α-synuclein in brain interstitial fluid. We show for the first time that α-synuclein is readily detected in the interstitial fluid of both α-synuclein transgenic mice and human patients with traumatic brain injury. Our data suggest that α-synuclein is physiologically secreted by neurons in vivo. This interstitial fluid pool of the protein may have a role in the propagation of synuclein pathology and progression of Parkinson's disease.

PMID:
21779395
PMCID:
PMC3136497
DOI:
10.1371/journal.pone.0022225
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center