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Methods Mol Biol. 2011;777:87-97. doi: 10.1007/978-1-61779-252-6_6.

αβ-Tubulin and microtubule-binding assays.

Author information

1
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC, USA.

Abstract

Dynamic instability is a hallmark of the microtubule cytoskeleton. In order to regulate microtubule dynamics in vivo, a varied host of microtubule-associated proteins are mobilized to promote microtubule nucleation, growth, stabilization, catastrophe, depolymerization, rescue, and severing. To confer these various functions, cytoskeletal regulators have highly tuned affinities for tubulin, recognizing the unpolymerized αβ-tubulin heterodimer, dynamic microtubule lattice, stabilized microtubule lattice, or a combination therein. The protocols presented here probe αβ-tubulin and microtubule binding using gel filtration and cosedimentation, respectively.

PMID:
21773922
DOI:
10.1007/978-1-61779-252-6_6
[Indexed for MEDLINE]

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