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Methods Mol Biol. 2011;777:15-28. doi: 10.1007/978-1-61779-252-6_2.

Purification of tubulin from porcine brain.

Author information

1
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. gell@mpi-cbg.de

Abstract

Microtubules, polymers of the heterodimeric protein αβ-tubulin, give shape to cells and are the tracks for vesicle transport and chromosome segregation. In vitro assays to study microtubule functions and their regulation by microtubule-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.

PMID:
21773918
DOI:
10.1007/978-1-61779-252-6_2
[Indexed for MEDLINE]

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