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Asian Pac J Trop Med. 2011 Jun;4(6):482-5. doi: 10.1016/S1995-7645(11)60131-4.

Inhibitive effect of triptolide on invasiveness of human fibrosarcoma cells by downregulating matrix metalloproteinase-9 expression.

Author information

1
Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha 410013, P.R. China.

Abstract

OBJECTIVE:

To explore the molecular mechanisms of antitumor properties of triptolide, a bioactive component isolated from the Chinese herb Tripterygium wolfordii Hook F.

METHODS:

Human fibrosarcoma HT-1080 cells were treated with different doses of triptolide for 72 h. Then the expression and activity of matrix metalloproteinase (MMP)-2 and -9 were measured and the invasiveness of triptolide-treated HT-1080 cells was compared with that of anti-MMP-9-treated HT-1080 cells.

RESULTS:

18 nmol/L triptolide inhibited the gene expression and activity of MMP-9, but not those of MMP-2, in HT-1080 cells. In addition, both 18 nmol/L triptolide and 3 μg/mL anti-MMP-9 significantly reduced the invasive potential of HT-1080 cells, by about 50% and 35%, respectively, compared with the control. Whereas there was no significant difference between the effect of 18 nmol/L triptolide and that of anti-MMP-9 on invasive potential of HT-1080 cells.

CONCLUSIONS:

These data suggest that triptolide inhibits tumor cell invasion partly by reducing MMP-9 gene expression and activity.

PMID:
21771704
DOI:
10.1016/S1995-7645(11)60131-4
[Indexed for MEDLINE]
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