Clinical and histological healing of a new collagen matrix in combination with the coronally advanced flap for the treatment of Miller class-I recession defects: an experimental study in the minipig

J Clin Periodontol. 2011 Sep;38(9):847-55. doi: 10.1111/j.1600-051X.2011.01767.x. Epub 2011 Jul 19.

Abstract

Aim: To describe the histological and clinical outcomes of the use of a xenogeneic collagen matrix (CM) in combination with the coronally advanced flap (CAF) in the treatment of localized Miller class-I gingival recessions.

Material and methods: Gingival recession defects were surgically created on 12 minipigs. The defects were randomly treated with either the CAF procedure and the interposition of a CM (test) or the CAF alone (control). Clinical and histological outcomes at 1, 4 and 12 weeks were evaluated.

Results: Histometrically, in the test group, there was a shorter junctional epithelial dimension [2.26 (SD 0.23) mm] compared with the control [2.79 (SD 0.77) mm]. On the contrary, the amount of newly formed cementum was larger in the test group [1.08 (SD 0.41) mm] than in the control group [0.75 (SD 0.25) mm], although the differences were not statistically significant.

Conclusions: Both techniques rendered similar clinical outcomes, achieving complete root coverage at the end of the study. Nevertheless, the CM graft attained more tissue regeneration, characterized by a shorter epithelium and a larger new cementum formation. The use of a xenogeneic CM resulted in the incorporation of the xenograft within the adjacent host connective tissues in the absence of significant inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Collagen Type I / therapeutic use*
  • Connective Tissue / transplantation*
  • Dental Cementum / physiology*
  • Dental Enamel Proteins / therapeutic use
  • Female
  • Gingival Recession / surgery*
  • Guided Tissue Regeneration, Periodontal / methods*
  • Random Allocation
  • Regeneration*
  • Surgical Flaps
  • Swine
  • Swine, Miniature
  • Tissue Scaffolds

Substances

  • Collagen Type I
  • Dental Enamel Proteins
  • enamel matrix proteins