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Proc Natl Acad Sci U S A. 2011 Aug 2;108(31):12805-10. doi: 10.1073/pnas.1102298108. Epub 2011 Jul 18.

SPR-5 is a histone H3K4 demethylase with a role in meiotic double-strand break repair.

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1
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

Abstract

Regulation of histone methylation levels has long been implicated in multiple cellular processes, many of which involve transcription. Here, however, we report a unique role for the Caenorhabditis elegans histone demethylase SPR-5 in meiotic DNA double-strand break repair (DSBR). SPR-5 shows enzymatic activity toward H3K4me2 both in vitro and in the nematode germline, and spr-5 mutants show several phenotypes indicating a perturbation of DSBR, including increased p53-dependent germ cell apoptosis, increased levels of the DSBR marker RAD-51, and sensitivity toward DSB-inducing treatments. spr-5 mutants show no transcriptional misregulation of known DSBR involved genes. Instead, SPR-5 shows a rapid subcellular relocalization upon DSB-inducing treatment, which suggests that SPR-5 may function directly in DSBR.

PMID:
21768382
PMCID:
PMC3150895
DOI:
10.1073/pnas.1102298108
[Indexed for MEDLINE]
Free PMC Article
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