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Neurotoxicology. 2011 Oct;32(5):656-60. doi: 10.1016/j.neuro.2011.06.005. Epub 2011 Jul 8.

Biomarkers of organophosphorus (OP) exposures in humans.

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1
Department of Medicine, Division Medical Genetics, University of Washington, Seattle, WA 98195-7720, United States.

Abstract

There are ongoing events where aircraft engine lubricant containing tricresyl phosphates (TCPs) contaminates aircraft cabins. Some individuals have experienced tremors or other neurological symptoms that may last for many months following exposures. Mass spectrometric (MS) protocols are being developed to determine the percentage of "biomarker proteins" that are modified by such exposures, specifically on active site serines. Both plasma butyrylcholinesterase (BChE) and red cell acylpeptide hydrolase (APH) are readily inhibited by 2-(ortho-cresyl)-4H-1,3,2-benzodioxaphosphoran-2-one (CBDP) or phenyl saligenin cyclic phosphate (PSP) and have the potential to provide information about the level of exposure of an individual. We have developed immunomagnetic bead-based single-step purification protocols for both BChE and APH and have characterized the active site serine adducts of BChE by MS.

PMID:
21767566
PMCID:
PMC3279568
DOI:
10.1016/j.neuro.2011.06.005
[Indexed for MEDLINE]
Free PMC Article
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