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Mol Gen Genet. 1990 Sep;223(3):517-20.

Cauliflower mosaic virus P35S promoter activity in Escherichia coli.

Author information

1
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

Abstract

We present evidence that the cauliflower mosaic virus promoter P35S can direct expression of the bacterial neomycin phosphotransferase II (NPTII) gene in Escherichia coli. Transcription is initiated at several sites, the major one being located approximately 315 bases upstream of the plant start site. The nucleotide sequence directly preceding this start site is strongly homologous to the prokaryotic promoter consensus sequence. Thus constructs designed for introduction into plants can be expressed in E. coli.

PMID:
2176717
DOI:
10.1007/bf00264462
[Indexed for MEDLINE]

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