Geographical location of bat capture sites in Spain. 1: A Coruña, 2: Lugo, 3: La Rioja, 4: Gerona, 5: Castellón, 6: Valencia, 7: Alicante, 8: Málaga, 9: Cádiz, 10: Sevilla, 11: Huelva, 12: Cáceres, 13: Menorca. The stars show the locations where positive samples were found

CoV phylogenetic reconstruction based on 396 bp of the RdRp gene including 14 Spanish CoV from different bat species and 77 alpha, beta and gammacoronaviruses obtained from GenBank. Accession numbers are shown in brackets. BatCoV detected in Spain are highlighted in italics. For the analyses GTR substitution model, gamma estimation and two simultaneous runs of 10⁷ generations were done, each with four Markov chains, and the trees were sampled every 100 generations. First 25% trees were excluded as burn-in from the analysis. Significant posterior probabilities are indicated. Complementary information about sequences used in this phylogenetic reconstruction are shown in Table . Positive samples described in this work are shown in shaded rectangles and ovals. The new alpha and betacoronavirus groups described in this work are shown in shaded ovals. Amino acid identity was calculated with MEGA 4 using the pairwise deletion option. The alignment comprised the same 396 bp of the RdRp gene used for the phylogenetic reconstruction. Amino acid identities across 132 amino acids are indicated next to the brackets that links every Spanish BatCoV to the phylogenetic neighbours