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Opt Lett. 2011 Jul 15;36(14):2728-30. doi: 10.1364/OL.36.002728.

Laboratory soft-x-ray microscope for cryotomography of biological specimens.

Author information

1
Biomedical and X-Ray Physics, Department of Applied Physics, KTH Royal Institute of Technology/AlbaNova, 10691 Stockholm, Sweden.

Abstract

Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a λ=2.48 nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, a 30 nm zone-plate objective, and a cryotilt sample holder. We demonstrate high-resolution imaging, as well as quantitative tomographic imaging, of frozen intact cells. The reconstructed tomogram of the intracellular local absorption coefficient shows details down to ∼100 nm.

PMID:
21765523
DOI:
10.1364/OL.36.002728
[Indexed for MEDLINE]

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