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Nat Methods. 2011 Jul 17;8(9):757-60. doi: 10.1038/nmeth.1652.

Deep and fast live imaging with two-photon scanned light-sheet microscopy.

Author information

1
California Institute of Technology, Beckman Institute, Pasadena, CA, USA.tvt@caltech.edu

Abstract

We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.

PMID:
21765409
DOI:
10.1038/nmeth.1652
[Indexed for MEDLINE]

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