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Trans R Soc Trop Med Hyg. 2011 Oct;105(10):598-600. doi: 10.1016/j.trstmh.2011.06.004. Epub 2011 Jul 20.

Survival of Burkholderia pseudomallei in distilled water for 16 years.

Author information

1
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand.

Abstract

Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10(7) cells/ml and 1.4×10(5) cells/ml, respectively. A colony count on agar of 1.0×10(6) cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement.

PMID:
21764093
PMCID:
PMC3183224
DOI:
10.1016/j.trstmh.2011.06.004
[Indexed for MEDLINE]
Free PMC Article

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