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Biochim Biophys Acta. 2011 Dec;1810(12):1340-5. doi: 10.1016/j.bbagen.2011.06.023. Epub 2011 Jul 6.

Streptonigrin inhibits β-Catenin/Tcf signaling and shows cytotoxicity in β-catenin-activated cells.

Author information

1
Department of Applied Chemistry, Dongduk Women's University, Seoul 136-714, Republic of Korea. sypark21@dongduk.ac.kr

Abstract

BACKGROUND:

Activation of β-catenin/T-cell factor (Tcf) signaling plays a role in human carcinogenesis. This suggests a possibility that the β-catenin/Tcf signaling activated by the accumulation of β-catenin in the nucleus is related to some type of human carcinogenesis. Therefore, if β-catenin's transcriptional activity can be markedly down-regulated, tumor growth will be suppressed in β-catenin activated types of cancer.

METHODS:

To investigate the activation or suppression of β-catenin/Tcf transcription, we established a transiently transfected cell line with a constitutively active β-catenin mutant gene whose product is not degraded. This cell line was also co-transfected with luciferase reporter gene constructs containing either an optimized (TOPflash) or mutant (FOPflash) Tcf-binding element.

RESULTS:

We identified the inhibitory effect of streptonigrin against β-catenin/Tcf signaling in β-catenin activated cells. Streptonigrin inhibited the transcriptional activity of β-catenin/Tcf in SW480 cells and HEK293 cells transiently transfected with a constitutively active mutant β-catenin gene. The growth inhibitory effect of streptonigrin was more evident in β-catenin-activated cancer cells than in non-activated cancer cells. The electrophoresis mobility shift assay showed that the binding of Tcf complexes with their specific DNA-binding sites was suppressed by streptonigrin.

CONCLUSION:

Streptonigrin is a negative regulator of β-catenin/Tcf signaling, and their inhibitory mechanism is related to the proliferation inhibitory effect on β-catenin-activated cancer cells.

GENERAL SIGNIFICANCE:

This report reveals a molecular mechanism underlying the anti-tumor effect of streptonigrin from the perspective β-catenin/Tcf signaling. Given its function in inhibiting β-catenin/Tcf signaling, streptonigrin may be of interest as a leading compound for chemotherapeutic agent against β-catenin-activated tumorigenesis.

PMID:
21763403
DOI:
10.1016/j.bbagen.2011.06.023
[Indexed for MEDLINE]

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