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Curr Top Microbiol Immunol. 2012;357:19-40. doi: 10.1007/82_2011_154.

How ricin and Shiga toxin reach the cytosol of target cells: retrotranslocation from the endoplasmic reticulum.

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1
School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK. R.A.Spooner@warwick.ac.uk

Abstract

A number of protein toxins bind at the surface of mammalian cells and after endocytosis traffic to the endoplasmic reticulum, where the toxic A chains are liberated from the holotoxin. The free A chains are then dislocated, or retrotranslocated, across the ER membrane into the cytosol. Here, in contrast to ER substrates destined for proteasomal destruction, they undergo folding to a catalytic conformation and subsequently inactivate their cytosolic targets. These toxins therefore provide toxic probes for testing the molecular requirements for retrograde trafficking, the ER processes that prepare the toxic A chains for transmembrane transport, the dislocation step itself and for the post-dislocation folding that results in catalytic activity. We describe here the dislocation of ricin A chain and Shiga toxin A chain, but also consider cholera toxin which bears a superficial structural resemblance to Shiga toxin. Recent studies not only describe how these proteins breach the ER membrane, but also reveal aspects of a fundamental cell biological process, that of ER-cytosol dislocation.

PMID:
21761287
DOI:
10.1007/82_2011_154
[Indexed for MEDLINE]
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