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Neurosurgery. 2012 Jan;70(1):198-204; discussion 204. doi: 10.1227/NEU.0b013e31822ce963.

Retroviral delivery of platelet-derived growth factor to spinal cord progenitor cells drives the formation of intramedullary gliomas.

Author information

1
Department of Neurological Surgery, Columbia University Medical Center, New York, New York 10032, USA. jae2109@columbia.edu

Abstract

BACKGROUND:

High-grade gliomas of the spinal cord are poorly understood tumors that are very commonly associated with bad outcomes. The transforming effects of platelet-derived growth factor (PDGF) on spinal cord glial progenitor cells may play an important role in the development of these tumors.

OBJECTIVE:

To investigate the possible tumor-initiating effects of PDGF overexpression in the spinal cord, we delivered a PDGF retrovirus directly into the substance of the spinal cord.

METHODS:

The spinal cords of wild-type adult rats were surgically exposed and injected with 10⁶ colony-forming units of a green fluorescent protein-tagged, PDGF-expressing retrovirus. A control virus was injected to assess the cell types that become infected during retroviral delivery to the spinal cord.

RESULTS:

It was observed that PDGF overexpression in the spinal cord causes morbidity from high-grade intramedullary glioma formation between 27 and 49 days after PDGF retrovirus injection. Retroviral transduction was highly efficient with 100% of injected animals displaying the tumor phenotype. The tumors produced were highly proliferative, were locally invasive, and displayed the immunophenotype of virus-targeted glial progenitor cells (Olig2+PDGFR+NG2+GFAP-).

CONCLUSION:

PDGF is capable of driving glial progenitor cells within the adult spinal cord to form high-grade gliomas. Further investigation of PDGF signaling in the spinal cord is needed to better understand and treat these devastating tumors.

PMID:
21760556
PMCID:
PMC3869993
DOI:
10.1227/NEU.0b013e31822ce963
[Indexed for MEDLINE]
Free PMC Article

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