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J Gen Virol. 2011 Nov;92(Pt 11):2542-8. doi: 10.1099/vir.0.034983-0. Epub 2011 Jul 13.

Cyclosporin A inhibits the replication of diverse coronaviruses.

Author information

1
Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.

Abstract

Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.

PMID:
21752960
PMCID:
PMC3352363
DOI:
10.1099/vir.0.034983-0
[Indexed for MEDLINE]
Free PMC Article

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