A) BSK-lite containing GlcNAc as the sole carbohydrate. •, B31-A3; □, CP176. Left panel, 25°C: B31-A3 achieved a stationary phase cell concentration of 1.8×108 and CP176 achieved a stationary phase cell concentration of 1.5×108. Right panel, 37°C: B31-A3 achieved a stationary phase cell concentration of 8.5×107 and CP176 achieved a stationary phase cell concentration of 6.3×107. All data points were determined in quadruplicate. Results are representative of two independent experiments. B) BSK-lite containing GlcNAc and glycerol as the principal carbohydrate. •, B31-A3; □, CP176. Left panel, 25°C: B31-A3 achieved a stationary phase cell concentration of 6.4×108 and CP176 achieved a stationary phase cell concentration of 1.1×108. The difference in stationary phase cell concentration between B31-A3 and CP176 at 25°C was significant (P<.001). Right panel, 37°C: B31-A3 achieved a stationary phase cell concentration of 9.1×107 and CP176 achieved a stationary phase cell concentration of 6.4×107. All data points were measured in quadruplicate. Results are representative of three independent experiments. Error bars indicate SEM.