Format

Send to

Choose Destination
Methods Mol Biol. 2011;769:387-402. doi: 10.1007/978-1-61779-207-6_26.

Fluorescence recovery after photobleaching.

Author information

1
Faculty of Life Sciences, The University of Manchester, Manchester, UK.

Abstract

This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery.

PMID:
21748690
DOI:
10.1007/978-1-61779-207-6_26
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center