Genome-scale RNAi screening identifies essential genes in 102 cancer cell lines. (A) Chart showing the number of cell lines from different lineages screened. (B) Unsupervised hierarchical clustering of the shRNA hybridization data obtained from quadruplicate screens of 102 cancer cell lines (various colors) and the shRNA plasmid DNA reference pool (15 replicates). A representative portion of the dendrogram is depicted at higher magnification. (C) The relative abundance 7 d after infection of OVCAR-8 cells infected with 350 individual shRNAs encoded in a GFP+ plasmid (y axis) correlates with the relative abundance (log₂ fold change) of each shRNA measured in the pooled shRNA screen by microarray hybridization (x axis).

Dependencies of cell lines with mutations in KRAS, BRAF, or PIK3CA. (A, D, and G) Distribution of shRNA ranks (x axis) by the WoE scores (y axis) for the class comparisons of KRAS mutant (mut) vs. KRAS wild-type (wt) cell lines (A), BRAF mutant vs. BRAF wt (D), and PIK3CA mutant vs. PIK3CA wt (G). shRNAs targeting KRAS, BRAF, and PIK3CA are highlighted in red, and their ranks are listed. Insets report the gene ranks of KRAS, BRAF, and PIK3CA for differential essentiality in the subset of cell lines with activating mutations in those respective genes. (B, E, and H) KRAS (B), BRAF (E), or PIK3CA (H) mutation status (mutant lines in green, wt lines in gray) correlates strongly with depletion of shRNAs targeting these genes. (Lower) Heatmaps report KRAS- (B), BRAF- (E), and PIK3CA-shRNA (H) fold depletion in each cell line. (C, F, and I) Subsets of the 102 cell lines were analyzed to assess convergence of the gene dependency results for the KRAS (C), BRAF (F), and PIK3CA (I) mutant vs. wt class comparison analyses as a function of the number of cell lines tested. Distributions of the scores of the top KRAS, BRAF, and PIK3CA hit shRNAs (given as the percentile of their depletion rankings, with a smaller percentage corresponding to more depleted, y axis) in the respective cell line class comparisons (using WoE) are shown for each of 100 trials, subsampling the indicated numbers of cell lines in each class (mutant and wt). The red bar indicates the median value for each group of subsamplings, boxes represent the 25th to 75th percentile of the data, and whiskers extend to the most extreme values of the group that are not considered outliers.

Lineage-specific dependencies. (A) Heatmap of differentially antiproliferative shRNAs in cell lines from individual cancer lineages in comparison with all others. The top 20 shRNAs that distinguish each lineage from the others are displayed. (B) Ovarian-specific dependencies. Three complementary methods of gene scoring [ranking by (i) best or (ii) second best scoring shRNA or (iii) composite of all shRNAs for the gene using a KS statistic identified 582 (5.2%) genes that were selectively required for ovarian cancer cell proliferation. Fifty of these were among the 1,825 recurrently amplified genes in primary high-grade serous ovarian tumors (). Among the 200 genes that were differentially overexpressed in ovarian cancer cell lines, 114 genes were included in the shRNA pool, and 5 genes showed enhanced essentiality in ovarian cancer lines. Twenty-two of these genes that were scored by all three gene-scoring methods were considered as high-confidence essential genes. (C) Distributions of the scores of PAX8 shRNA (given as the percentile of their rankings, y axis) after 100 trials of the ovarian vs. nonovarian WoE comparison, for equal class sizes of 1–25 (x axis; colors indicate PAX8 shRNAs 1–5). The red bar indicates the median value for each group of subsamplings, boxes represent the 25th to 75th percentile of the data, and whiskers extend to the most extreme values of the group that are not considered outliers.

PAX8 is essential for ovarian cancer cell proliferation and survival. (A) PAX8 is the top-ranked differentially expressed gene between ovarian and nonovarian cancer cell lines. Arrow indicates PAX8. (B) SNP array colorgrams depict genomic amplification of PAX8 in primary high-grade serous ovarian cancers (). Regions of genomic amplification and deletion are denoted in red and blue, respectively. SNP array profiles derived from primary ovarian tumors were sorted based on the degree of amplification of each gene. Black vertical lines denote the boundaries of the PAX8 gene. (C) Boxplot showing significant difference in the degree of depletion of a PAX8-specific shRNA in 63 cell lines with low levels of PAX8 compared with 20 lines with high levels of PAX8 (P = 2.14 × 10⁻⁸, t test). Cell lines were divided into high- and low-expressing groups. The red line indicates the median value for each group, boxes represent the 25th to 75th percentile of the data, and whiskers extend to the most extreme values of the group that are not considered outliers. Ovarian cancer cell lines are plotted with red circles; cell lines from all other lineages are plotted with green circles. (D Upper) Effects of PAX8 suppression on proliferation in eight ovarian cancer cell lines; dotted line indicates 50% relative proliferation. (Lower) Immunoblot of PAX8 in a panel of eight ovarian cancer cell lines and in immortalized IOSE-T80 cells. Cell lines with amplification of 2q13 (log₂ copy number ratio > 0.3) are marked in red. * denotes nonspecific band. (E Upper) Effects of PAX8 suppression on proliferation of cell lines from indicated cancer types. (Lower) Immunoblot of PAX8. Error bars indicate SD of six replicate measurements. (F) Immunoblot of poly(ADP-ribose) polymerase after PAX8 suppression in two 2q13-amplified cell lines. * denotes nonspecific band.