Format

Send to

Choose Destination
Nat Neurosci. 2011 Jul 10;14(8):1081-8. doi: 10.1038/nn.2868.

High-accuracy neurite reconstruction for high-throughput neuroanatomy.

Author information

1
Max Planck Institute for Medical Research, Heidelberg, Germany. moritz.helmstaedter@mpimf-heidelberg.mpg.de

Abstract

Neuroanatomic analysis depends on the reconstruction of complete cell shapes. High-throughput reconstruction of neural circuits, or connectomics, using volume electron microscopy requires dense staining of all cells, which leads even experts to make annotation errors. Currently, reconstruction speed rather than acquisition speed limits the determination of neural wiring diagrams. We developed a method for fast and reliable reconstruction of densely labeled data sets. Our approach, based on manually skeletonizing each neurite redundantly (multiple times) with a visualization-annotation software tool called KNOSSOS, is ∼50-fold faster than volume labeling. Errors are detected and eliminated by a redundant-skeleton consensus procedure (RESCOP), which uses a statistical model of how true neurite connectivity is transformed into annotation decisions. RESCOP also estimates the reliability of consensus skeletons. Focused reannotation of difficult locations promises a rather steep increase of reliability as a function of the average skeleton redundancy and thus the nearly error-free analysis of large neuroanatomical datasets.

PMID:
21743472
DOI:
10.1038/nn.2868
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center