Format

Send to

Choose Destination
See comment in PubMed Commons below
Biotechnol Adv. 2012 Jan-Feb;30(1):99-107. doi: 10.1016/j.biotechadv.2011.06.014. Epub 2011 Jun 29.

Simulation of the Ras/cAMP/PKA pathway in budding yeast highlights the establishment of stable oscillatory states.

Author information

1
Università degli Studi di Milano-Bicocca, Dipartimento di Statistica, Milano, Italy. dario.pescini@unimib.it

Abstract

In the yeast Saccharomyces cerevisiae, the Ras/cAMP/PKA pathway plays a major role in the regulation of metabolism, stress resistance and cell cycle progression. We extend here a mechanistic model of the Ras/cAMP/PKA pathway that we previously defined by describing the molecular interactions and post-translational modifications of proteins, and perform a computational analysis to investigate the dynamical behaviors of the components of this pathway, regulated by different control mechanisms. We carry out stochastic simulations to consider, in particular, the effect of the negative feedback loops on the activity of both Ira2 (a Ras-GAP) and Cdc25 (a Ras-GEF) proteins. Our results show that stable oscillatory regimes for the dynamics of cAMP can be obtained only through the activation of these feedback mechanisms, and when the amount of Cdc25 is within a specific range. In addition, we highlight that the levels of guanine nucleotides pools are able to regulate the pathway, by influencing the transition between stable steady states and oscillatory regimes.

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center