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Exp Dermatol. 2011 Oct;20(10):805-9. doi: 10.1111/j.1600-0625.2011.01326.x. Epub 2011 Jul 11.

Oral administration of Escherichia coli Nissle 1917 prevents allergen-induced dermatitis in mice.

Author information

1
Allergy-Center-Charité, Department of Dermatology and Allergy, Charité-Universitätsmedizin Berlin, Berlin, Germany.

Abstract

BACKGROUND:

The prevalence of allergies has been linked to Western life style factors including a decrease of microbial exposure. Probiotics, such as Escherichia coli Nissle 1917 (EcN), have been shown to be beneficial for prevention and treatment of several chronic inflammatory diseases.

OBJECTIVE:

The aim of this study was to investigate the impact of oral EcN administration on development and outcome of allergen-induced dermatitis.

METHODS:

In sensitized BALB/c mice, skin inflammation was induced by topical allergen application. EcN was administered orally in a preventive manner. Severity of dermatitis was analysed by evaluation of skin score, local cellular and cytokine profile. The systemic immune response was assessed by analysis of immunoglobulins and allergen-dependent cytokine response.

RESULTS:

Oral EcN administration improved allergen-induced dermatitis dose-dependently. In parallel, a reduction of epidermal thickness and infiltrating immune cells together with an enhanced number of forkhead box P3 (Foxp3)(+) cells and a trend of increased IFNγ, IL-10 and TGFβ expression was detected in eczematous skin. In allergen-stimulated splenocytes reduced IL-4 and IFNγ along with an elevated IL-10 production and a tendency to an increased TGFβ secretion were observed.

CONCLUSIONS:

Our findings indicate that EcN alters the local allergen-induced immune response by increase of Foxp3(+) cells and by favouring an immunoregulatory cytokine pattern. Thus, oral administration of EcN might be an effective strategy in prevention and potentially therapy of allergic inflammatory skin diseases.

[Indexed for MEDLINE]

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