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Langmuir. 2011 Aug 16;27(16):10119-23. doi: 10.1021/la2022177. Epub 2011 Jul 14.

Preparation of peptide-functionalized gold nanoparticles using one pot EDC/sulfo-NHS coupling.

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1
School of Physics and Astronomy, University of Southampton, Southampton SO17 1BJ, UK.

Abstract

Although carbodiimides and succinimides are broadly employed for the formation of amide bonds (i.e., in amino acid coupling), their use in the coupling of peptides to water-soluble carboxylic-terminated colloidal gold nanoparticles remains challenging. In this article, we present an optimization study for the successful coupling of the KPQPRPLS peptide to spherical and rodlike colloidal gold nanoparticles. We show that the concentration, reaction time, and chemical environment are all critical to achieving the formation of robust, peptide-coated colloidal nanoparticles. Agarose gel electrophoresis was used for the characterization of conjugates.

PMID:
21728291
DOI:
10.1021/la2022177
[Indexed for MEDLINE]
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