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Nano Lett. 2011 Aug 10;11(8):3324-8. doi: 10.1021/nl201689v. Epub 2011 Jul 13.

Dynamic and reversible control of 2D membrane protein concentration in a droplet interface bilayer.

Author information

1
Department of Chemistry, Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, OX1 3TA, Oxford, U.K.

Abstract

We form an artificial lipid bilayer between a nanolitre aqueous droplet and a supporting hydrogel immersed in an oil/lipid solution. Manipulation of the axial position of the droplet relative to the hydrogel controls the size of the bilayer formed at the interface; this enables the surface density of integral membrane proteins to be controlled. We are able to modulate the surface density of the β-barrel pore-forming toxin α-hemolysin over a range of 4 orders of magnitude within a time frame of a few seconds. The concentration changes are fully reversible. Membrane protein function and diffusion are unaltered, as measured by single molecule microscopy and single channel electrical recording.

PMID:
21726072
DOI:
10.1021/nl201689v
[Indexed for MEDLINE]

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