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Nat Methods. 2011 Jul 3;8(8):649-51. doi: 10.1038/nmeth.1640.

Megapixel digital PCR.

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1
Centre for High-Throughput Biology, University of British Columbia, Vancouver, British Columbia, Canada.

Abstract

We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.

PMID:
21725299
DOI:
10.1038/nmeth.1640
[Indexed for MEDLINE]
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