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Biophys J. 2011 Jul 6;101(1):43-52. doi: 10.1016/j.bpj.2011.05.044.

Cell cytoskeleton and tether extraction.

Author information

1
Laboratório de Pinças Óticas da Coordenação de Programas de Estudos Avançados, Instituto de Ciências Biomédicas, Rio de Janeiro, Brazil.

Abstract

We perform a detailed investigation of the force × deformation curve in tether extraction from 3T3 cells by optical tweezers. Contrary to conventional wisdom about tethers extracted from cells, we find that actin filaments are present within them, so that a revised theory of tether pulling from cells is called for. We also measure steady and maximum tether force values significantly higher than previously published ones for 3T3 cells. Possible explanations for these differences are investigated. Further experimental support of the theory of force barriers for membrane tube extension is obtained. The potential of studies on tether pulling force × deformation for retrieving information on membrane-cytoskeleton interaction is emphasized.

PMID:
21723813
PMCID:
PMC3127177
DOI:
10.1016/j.bpj.2011.05.044
[Indexed for MEDLINE]
Free PMC Article

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