Cervical precancerous lesions--chromosomal instability in peripheral blood lymphocytes in relation to lesion stage, age and smoking habits

Acta Obstet Gynecol Scand. 2011 Oct;90(10):1082-7. doi: 10.1111/j.1600-0412.2011.01230.x. Epub 2011 Aug 26.

Abstract

Objective: To evaluate chromosomal damage in peripheral blood lymphocytes (PBL) of patients newly diagnosed with cervical precancerous lesions with respect to age, smoking habits, miscarriages, abortions and lesion stage.

Design: Clinical study.

Setting: Clinic of Gynecology and Obstetrics in Kragujevac, Serbia, during 2009-2010.

Population: The analyzed samples included 41 untreated patients aged 24-65 years with a diagnosed low-grade squamous intraepithelial lesion (LSIL; 19 patients) or a high-grade squamous intraepithelial lesion (HSIL; 22 patients). Control samples were obtained from 40 healthy women aged 24-53 years.

Methods: The frequency of micronuclei (MN) was estimated in circulating lymphocytes by using the cytokinesis-block micronucleus assay.

Main outcome measure: The frequency of MN in PBL.

Results: The mean MN frequencies of both LSIL and HSIL patients were significantly higher than the MN frequency of healthy control women. There was no significant difference in MN frequency between LSIL and HSIL patients, between smokers and nonsmokers in both patient and control samples, or between miscarriage groups and abortion groups of patients. Considering confounder factors, age and health status influenced MN frequency.

Conclusions: The results suggest that MN frequency in PBL of patients with cervical precancerous lesions corresponds to an increase of chromosomal damage, irrespective of smoking habits, miscarriages, abortions and lesion stages.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Age Factors
  • Aged
  • Analysis of Variance
  • Carcinoma, Squamous Cell / epidemiology
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / pathology*
  • Case-Control Studies
  • Chromosomal Instability
  • Female
  • Humans
  • Incidence
  • Linear Models
  • Lymphocytes / pathology
  • Micronuclei, Chromosome-Defective*
  • Micronucleus Tests
  • Middle Aged
  • Multivariate Analysis
  • Neoplasm Staging
  • Precancerous Conditions / pathology*
  • Prognosis
  • Reference Values
  • Risk Factors
  • Smoking / adverse effects
  • Uterine Cervical Dysplasia / epidemiology
  • Uterine Cervical Dysplasia / genetics
  • Uterine Cervical Dysplasia / pathology
  • Uterine Cervical Neoplasms / epidemiology
  • Uterine Cervical Neoplasms / genetics
  • Uterine Cervical Neoplasms / pathology
  • Young Adult