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Macromol Rapid Commun. 2009 May 19;30(9-10):872-7. doi: 10.1002/marc.200800747. Epub 2009 Mar 17.

Optical waveguide spectroscopy for the investigation of protein-functionalized hydrogel films.

Author information

1
Max-Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.

Abstract

This article reports the implementation of optical waveguide spectroscopy (OWS) for the quantitative time-resolved observation of changes in the swelling behavior and mass density of protein-functionalized hydrogel films. In the experiment, a thin film of an N-isopropylacrylamide (NIPAAm)-based polymer that supported optical waveguide modes is attached to a metallic sensor surface. IgG molecules are in situ immobilized in this gel by using novel coupling chemistry with a charge-attraction scheme based on a tetrafluorophenol sulfonate active ester. The anti-fouling properties of the functionalized hydrogel network and the kinetics of the affinity binding of protein molecules in the gel are investigated.

PMID:
21706671
DOI:
10.1002/marc.200800747

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