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Biophys Chem. 2011 Nov;159(1):120-8. doi: 10.1016/j.bpc.2011.05.014. Epub 2011 May 27.

The use of analytical sedimentation velocity to extract thermodynamic linkage.

Author information

1
Department of Molecular and Cell Biology, University of Connecticut Storrs, CT 06269, USA.

Abstract

For 25 years, the Gibbs Conference on Biothermodynamics has focused on the use of thermodynamics to extract information about the mechanism and regulation of biological processes. This includes the determination of equilibrium constants for macromolecular interactions by high precision physical measurements. These approaches further reveal thermodynamic linkages to ligand binding events. Analytical ultracentrifugation has been a fundamental technique in the determination of macromolecular reaction stoichiometry and energetics for 85 years. This approach is highly amenable to the extraction of thermodynamic couplings to small molecule binding in the overall reaction pathway. In the 1980s this approach was extended to the use of sedimentation velocity techniques, primarily by the analysis of tubulin-drug interactions by Na and Timasheff. This transport method necessarily incorporates the complexity of both hydrodynamic and thermodynamic nonideality. The advent of modern computational methods in the last 20 years has subsequently made the analysis of sedimentation velocity data for interacting systems more robust and rigorous. Here we review three examples where sedimentation velocity has been useful at extracting thermodynamic information about reaction stoichiometry and energetics. Approaches to extract linkage to small molecule binding and the influence of hydrodynamic nonideality are emphasized. These methods are shown to also apply to the collection of fluorescence data with the new Aviv FDS.

PMID:
21703752
PMCID:
PMC3166974
DOI:
10.1016/j.bpc.2011.05.014
[Indexed for MEDLINE]
Free PMC Article

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