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Fertil Steril. 2011 Aug;96(2):439-44. doi: 10.1016/j.fertnstert.2011.05.062. Epub 2011 Jun 24.

Early growth response-2 expression in uterine leiomyoma cells: regulation and function.

Author information

1
Division of Reproductive Biology Research, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.

Abstract

OBJECTIVE:

To investigate the regulation of early growth response-2 (Egr-2) by transforming growth factor β3 (TGF-β3) and its functions in cultured human uterine leiomyoma smooth muscle cells.

DESIGN:

Laboratory research.

SETTING:

Academic medical center.

PATIENT(S):

Primary leiomyoma cells from patients with symptomatic leiomyomata.

INTERVENTION(S):

Tissue culture followed by RNA and protein analysis.

MAIN OUTCOME MEASURE(S):

Cell proliferation, alteration in extracellular matrix component expression.

RESULT(S):

In vivo mRNA levels of Egr-2 were statistically significantly higher in leiomyoma tissues compared with matched myometrial tissues, and showed a statistically significant correlation with TGF-β3 messenger RNA (mRNA) levels in leiomyoma tissues. In primary leiomyoma smooth muscle cells, TGF-β3 statistically significantly induced Egr-2 gene expression in a dose-dependent and time-dependent manner. Small interfering RNA (siRNA) knockdown of Egr-2 markedly increased the level of the proliferation marker proliferating cell nuclear antigen and the expression of proto-oncogene c-myc. On the other hand, ablation of Egr-2 stimulated collagen-1A1 and collagen-3A1 transcription and inhibited dermatopontin gene expression. However, the mRNA levels of α-smooth muscle actin and fibronectin were not affected by Egr-2 knockdown.

CONCLUSION(S):

We demonstrated that TGF-β3 regulated Egr-2 gene expression and presented evidence that Egr-2 decreases collagen production and stimulates dermatopontin gene expression.

PMID:
21703609
PMCID:
PMC3143242
DOI:
10.1016/j.fertnstert.2011.05.062
[Indexed for MEDLINE]
Free PMC Article

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