Format

Send to

Choose Destination
PLoS Genet. 2011 Jun;7(6):e1001392. doi: 10.1371/journal.pgen.1001392. Epub 2011 Jun 9.

A large gene network in immature erythroid cells is controlled by the myeloid and B cell transcriptional regulator PU.1.

Author information

1
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, United States of America.

Abstract

PU.1 is a hematopoietic transcription factor that is required for the development of myeloid and B cells. PU.1 is also expressed in erythroid progenitors, where it blocks erythroid differentiation by binding to and inhibiting the main erythroid promoting factor, GATA-1. However, other mechanisms by which PU.1 affects the fate of erythroid progenitors have not been thoroughly explored. Here, we used ChIP-Seq analysis for PU.1 and gene expression profiling in erythroid cells to show that PU.1 regulates an extensive network of genes that constitute major pathways for controlling growth and survival of immature erythroid cells. By analyzing fetal liver erythroid progenitors from mice with low PU.1 expression, we also show that the earliest erythroid committed cells are dramatically reduced in vivo. Furthermore, we find that PU.1 also regulates many of the same genes and pathways in other blood cells, leading us to propose that PU.1 is a multifaceted factor with overlapping, as well as distinct, functions in several hematopoietic lineages.

PMID:
21695229
PMCID:
PMC3111485
DOI:
10.1371/journal.pgen.1001392
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center