Zn(II) and Cd(II)-based complexes for probing the enzymatic hydrolysis of Na4P2O7 by alkaline phosphatase in physiological conditions

Priyadip Das; Sourish Bhattacharya; Sandhya Mishra; Amitava Das

doi:10.1039/c1cc12682b

Zn(II) and Cd(II)-based complexes for probing the enzymatic hydrolysis of Na4P2O7 by alkaline phosphatase in physiological conditions

Chem Commun (Camb). 2011 Jul 28;47(28):8118-20. doi: 10.1039/c1cc12682b. Epub 2011 Jun 17.

Authors

Priyadip Das¹, Sourish Bhattacharya, Sandhya Mishra, Amitava Das

Affiliation

¹ Central Salt & Marine Chemicals Research Institute (CSIR), Bhavnaga, 364002, Gujarat, India.

PMID: 21687847
DOI: 10.1039/c1cc12682b

Abstract

Newly synthesized Zn(II) and Cd(II)-based complexes show unique selectivity towards inorganic pyrophosphate (PPi) in 100% aqueous medium at pH = 7.4 and act as a "turn-on" and "turn-off" real-time assay, respectively, for the evaluation of the enzymatic activity of alkaline phosphatase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaline Phosphatase / metabolism*
Cadmium / chemistry*
Diphosphates / metabolism*
Enzyme Assays / methods*
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Humans
Hydrogen-Ion Concentration
Hydrolysis
Organometallic Compounds / chemical synthesis
Organometallic Compounds / chemistry*
Zinc / chemistry*

Substances

Diphosphates
Fluorescent Dyes
Organometallic Compounds
Cadmium
Alkaline Phosphatase
Zinc
sodium pyrophosphate