Nerve growth factor induced expression of iNOS and substance P in dorsal root ganglion sensory neuron and interferon regulatory factor-1

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 May;36(5):386-91. doi: 10.3969/j.issn.1672-7347.2011.05.003.

Abstract

Objective: To investigate the mechanism of airway neurogenic inflammation by studying the expression of inducible nitric oxide synthase (iNOS) and substance P in C(7)-T(5) dorsal root ganglion sensory neuron cells induced by nerve growth factor (NGF) and the role of interferon regulatory factor-1 (IRF-1).

Methods: The dorsal root ganglia neuron (DRGn) cells were primary cultured, and then stimulated with or without NGF or NGF+interferon (IFN)-γ. Subsequently the DRGn cells were transinfected with or without green fluorescent protein (GFP)-IRF-1-vshRNA, and then stimulated with or without NGF. The expressions of iNOS and substance P were detected by real-time PCR.

Results: NGF induced the mRNA expression of iNOS and substance P in dorsal root ganglion sensory neuron cells, and IFN-γ increased NGF-induced iNOS mRNA expression. The expressions of iNOS and substance P in sensory neuron cells were decreased significantly at the mRNA level after IRF-1 was blocked down by IRF-1-vshRNA transinfection.

Conclusion: NGF is involved in the airway neurogenic inflammation by prompting the expression of iNOS and substance P through transcription factor IRF-1 in airway sensory neuron cells. IRF-1 may be a therapeutic target for airway neurogenic inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Embryo, Mammalian
  • Female
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / metabolism*
  • Inflammation
  • Interferon Regulatory Factor-1 / metabolism*
  • Nerve Growth Factor / pharmacology*
  • Nitric Oxide Synthase Type II / genetics
  • Nitric Oxide Synthase Type II / metabolism*
  • Pregnancy
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Respiratory System / cytology
  • Sensory Receptor Cells / metabolism
  • Substance P / genetics
  • Substance P / metabolism*
  • Transfection

Substances

  • Interferon Regulatory Factor-1
  • RNA, Messenger
  • Substance P
  • Nerve Growth Factor
  • Nitric Oxide Synthase Type II
  • Nos2 protein, rat