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Bioorg Med Chem Lett. 2011 Jul 15;21(14):4206-9. doi: 10.1016/j.bmcl.2011.05.084. Epub 2011 May 30.

BODIPY-modified 2'-deoxyguanosine as a novel tool to detect DNA damages.

Author information

1
Department of Applied Chemistry, Kanagawa Institute of Technology, Shimo-Ogino 1030, Atsugi-shi 243-0292, Japan. takamura@chem.kanagawa-it.ac.jp

Abstract

BODIPY-modified 2'-deoxyguanosine was synthesized for use as a detection reagent for genotoxic compounds. BODIPY-FL is a well known fluorescence reagent whose fluorescent light emission diminishes near a guanine base by a photo-induced electron transfer process. We attached BODIPY-Fl to the 5' position of the deoxyribose moiety of 2'-deoxyguanosine. Although this compound has low fluorescence activity, when depurination by the action of alkylating reagents and dG oxidation by singlet oxygen occurred, the emission of strong fluorescence was observed. BODIPY-dG was found, therefore, to be a very useful tool for selectively detecting DNA damaging activity particularly in natural environmental extracts.

PMID:
21684162
DOI:
10.1016/j.bmcl.2011.05.084
[Indexed for MEDLINE]

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