Format

Send to

Choose Destination
See comment in PubMed Commons below
Phys Med Biol. 2011 Jul 7;56(13):4013-21. doi: 10.1088/0031-9155/56/13/017. Epub 2011 Jun 15.

The refractive index of human hemoglobin in the visible range.

Author information

1
International Research-Educational Center of Optical Technologies for Industry and Medicine Photonics, Saratov State University, 83 Astrakhanskaya str., 410012 Saratov, Russia.

Abstract

Because the refractive index of hemoglobin in the visible range is sensitive to the hemoglobin concentration, optical investigations of hemoglobin are important for medical diagnostics and treatment. Direct measurements of the refractive index are, however, challenging; few such measurements have previously been reported, especially in a wide wavelength range. We directly measured the refractive index of human deoxygenated and oxygenated hemoglobin for nine wavelengths between 400 and 700 nm for the hemoglobin concentrations up to 140 g l(-1). This paper analyzes the results and suggests a set of model functions to calculate the refractive index depending on the concentration. At all wavelengths, the measured values of the refractive index depended on the concentration linearly. Analyzing the slope of the lines, we determined the specific refraction increments, derived a set of model functions for the refractive index depending on the concentration, and compared our results with those available in the literature. Based on the model functions, we further calculated the refractive index at the physiological concentration within the erythrocytes of 320 g l(-1). The results can be used to calculate the refractive index in the visible range for arbitrary concentrations provided that the refractive indices depend on the concentration linearly.

PMID:
21677368
DOI:
10.1088/0031-9155/56/13/017
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for IOP Publishing Ltd.
    Loading ...
    Support Center