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J Clin Microbiol. 2011 Aug;49(8):3068-70. doi: 10.1128/JCM.00718-11. Epub 2011 Jun 15.

Cepheid GeneXpert MTB/RIF assay for Mycobacterium tuberculosis detection and rifampin resistance identification in patients with substantial clinical indications of tuberculosis and smear-negative microscopy results.

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1
National Reference Laboratory for Mycobacteria (NRLM), Sotiria Chest Diseases Hospital, Mesogion 152, 11527 Athens, Greece. panayotisioannidis@gmail.com

Abstract

The GeneXpert MTB/RIF assay was evaluated with microscopically negative and positive pulmonary and extrapulmonary specimens from patients with substantial clinical indications for tuberculosis. For the pulmonary samples, the sensitivity, specificity, and positive and negative predictive values were 90.6%, 94.3%, 93.5%, and 91.7%, and for the extrapulmonary samples, they were 100%, 91.6%, 50%, and 100%, respectively. For microscopically negative specimens, the respective values were 86.3%, 93%, 79%, and 95.6%. The assay correctly detected rifampin resistance in all but one specimen, which harbored a mixed population. The GeneXpert assay was highly effective for tuberculosis diagnosis and identification of rifampin-resistant strains in smear-negative samples.

PMID:
21677069
PMCID:
PMC3147726
DOI:
10.1128/JCM.00718-11
[Indexed for MEDLINE]
Free PMC Article
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