Format

Send to

Choose Destination
See comment in PubMed Commons below
Curr Opin Chem Biol. 2011 Oct;15(5):733-40. doi: 10.1016/j.cbpa.2011.05.016. Epub 2011 Jun 12.

Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry.

Author information

1
School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7DN, UK. John.Fletcher@manchester.ac.uk

Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides a method for the detection of native and exogenous compounds in biological samples on a cellular scale. Through the development of novel ion beams the amount of molecular signal available from the sample surface has been increased. Through the introduction of polyatomic ion beams, particularly C(60), ToF-SIMS can now be used to monitor molecular signals as a function of depth as the sample is eroded thus proving the ability to generate 3D molecular images. Here we describe how this new capability has led to the development of novel instrumentation for 3D molecular imaging while also highlighting the importance of sample preparation and discuss the challenges that still need to be overcome to maximise the impact of the technique.

PMID:
21664172
PMCID:
PMC3181283
DOI:
10.1016/j.cbpa.2011.05.016
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center