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J Microbiol Methods. 2011 Sep;86(3):376-8. doi: 10.1016/j.mimet.2011.05.020. Epub 2011 Jun 2.

Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates.

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Swedish University of Agricultural Sciences, Department of Microbiology, Box 7025, SE-750 07 Uppsala, Sweden.


Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU.

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