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Methods Mol Biol. 2011;745:283-91. doi: 10.1007/978-1-61779-129-1_16.

Homologous recombination assay for interstrand cross-link repair.

Author information

1
Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA. nakanisk@mskcc.org

Abstract

DNA interstrand cross-links (ICLs) covalently link both strands of the DNA duplex, impeding cellular processes like DNA replication. Homologous recombination (HR) is considered to be a major pathway for the repair of ICLs in mammalian cells as mutants for HR components are highly sensitive to DNA-damaging agents that cause ICLs. This chapter describes GFP assays to measure HR following site-specific ICL formation with psoralen through DNA triplex technology. This approach can be used to determine the genetic requirements for ICL-induced HR in relation to those involved in HR repair of other DNA lesions such as double-strand breaks.

PMID:
21660700
PMCID:
PMC3261721
DOI:
10.1007/978-1-61779-129-1_16
[Indexed for MEDLINE]
Free PMC Article

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