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Circ Res. 2011 Jul 22;109(3):262-71. doi: 10.1161/CIRCRESAHA.111.244244. Epub 2011 Jun 9.

Desmoplakin and talin2 are novel mRNA targets of fragile X-related protein-1 in cardiac muscle.

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1
Department of Cellular and Molecular Medicine, University of Arizona, 1656 East Mabel, Tucson, AZ 85724, USA.

Abstract

RATIONALE:

The proper function of cardiac muscle requires the precise assembly and interactions of numerous cytoskeletal and regulatory proteins into specialized structures that orchestrate contraction and force transmission. Evidence suggests that posttranscriptional regulation is critical for muscle function, but the mechanisms involved remain understudied.

OBJECTIVE:

To investigate the molecular mechanisms and targets of the muscle-specific fragile X mental retardation, autosomal homolog 1 (FXR1), an RNA binding protein whose loss leads to perinatal lethality in mice and cardiomyopathy in zebrafish.

METHODS AND RESULTS:

Using RNA immunoprecipitation approaches we found that desmoplakin and talin2 mRNAs associate with FXR1 in a complex. In vitro assays indicate that FXR1 binds these mRNA targets directly and represses their translation. Fxr1 KO hearts exhibit an up-regulation of desmoplakin and talin2 proteins, which is accompanied by severe disruption of desmosome as well as costamere architecture and composition in the heart, as determined by electron microscopy and deconvolution immunofluorescence analysis.

CONCLUSIONS:

Our findings reveal the first direct mRNA targets of FXR1 in striated muscle and support translational repression as a novel mechanism for regulating heart muscle development and function, in particular the assembly of specialized cytoskeletal structures.

PMID:
21659647
PMCID:
PMC3163600
DOI:
10.1161/CIRCRESAHA.111.244244
[Indexed for MEDLINE]
Free PMC Article

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