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J Neurosci Methods. 2011 Aug 15;199(2):249-57. doi: 10.1016/j.jneumeth.2011.05.020. Epub 2011 May 31.

Enzyme-linked immunosorbent assays for α-synuclein with species and multimeric state specificities.

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1
Department of Anatomy, School of Medicine, Konkuk University, Seoul 143-701, South Korea.

Abstract

Abnormal intracellular deposition of aggregated α-synuclein is the characteristic feature of a number of neurological disorders, including Parkinson's disease (PD). Although α-synuclein is typically known as a cytosolic protein, a small amount is secreted by exocytosis in both monomeric and aggregated forms. The extracellular forms of α-synuclein in human body fluids, such as cerebrospinal fluid (CSF) and blood plasma, might be a diagnostic target for PD and related diseases. Here, we characterized a new set of monoclonal antibodies against α-synuclein, and using different combinations of antibodies, we established ELISA systems to specifically detect human α-synuclein, mouse and human α-synuclein together, and multimeric forms of α-synuclein in biological samples. By employing the Tyramide signal amplification method, the sensitivity of the assay was significantly improved to detect a concentration as low as ∼12.5 pg/ml. These assays might be useful tools for quantitative analysis of α-synuclein in various forms and with high sensitivity in diverse biological samples.

PMID:
21658411
DOI:
10.1016/j.jneumeth.2011.05.020
[Indexed for MEDLINE]

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