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Biotechnol Bioeng. 2011 Nov;108(11):2682-90. doi: 10.1002/bit.23226. Epub 2011 Jun 24.

2D format for screening bacterial cells at the throughput of flow cytometry.

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1
Institute of Protein Research of the Russian Academy of Sciences, 4 Institutskaya Str., Pushchino, Moscow Region 142290, Russia.

Abstract

We present a method for generating gel-based unordered 2D arrays of bacterial cells of a very high density, up to 10(5) cells per mm(2). Bacteria in a suspension are focused into a thin layer when the suspension and a dry gel matrix penetrate each other. Formation of a second gel from gel-forming components contained in the suspension results in immobilization of the cells. The immobilized cells stay alive and can repeatedly divide to produce microcolonies. The method provides for high-throughput screening and massively parallel analysis of individual cells in large populations, as well as for rapid isolation of rare clones.

PMID:
21656706
DOI:
10.1002/bit.23226
[Indexed for MEDLINE]

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