Format

Send to

Choose Destination
See comment in PubMed Commons below
Inhal Toxicol. 2011 Jun;23 Suppl 1:70-83. doi: 10.3109/08958378.2010.542580.

A comprehensive evaluation of the toxicology of cigarette ingredients: cocoa-derived ingredients.

Author information

1
Carson Watts Consulting, King, North Carolina, USA.

Abstract

CONTEXT:

Cocoa-derived ingredients are used in cigarette tobacco.

OBJECTIVE:

A battery of tests was used to compare toxicity of mainstream smoke from experimental cigarettes containing different added levels of cocoa-derived ingredients.

MATERIALS AND METHODS:

Five cocoa-derived ingredients chocolate (CH), cocoa (COC), cocoa-grand prix black (CGPB), cocoa nibs tincture (CNT) and cocoa shells extract (CSE) were added individually to experimental cigarettes at three different levels. Smoke from each of the experimental cigarette types was evaluated using analytical chemistry; in vitro cytotoxicity and mutagenicity testing were performed for four of the five compounds. For CH, COC and CNT, 90-day smoke inhalation studies were performed with 6-week recovery periods.

RESULTS:

No consistent changes were found in the analytical chemistry results. Results of the cytotoxicity and mutagenicity were unaffected by any of the ingredients. Two of the three inhalation studies showed very few differences between the groups. The inhalation study with COC showed several increases in mean histopathology severity scores in groups exposed to different levels of COC, compared with the controls. These apparent effects of COC on histopathology lesion severity scores were only present in a single sex and none were dose-related, which is not consistent with a true increase in biological activity. Also there were effectively no differences in the patterns of recovery for any of the compounds.

CONCLUSIONS:

Even at high inclusion levels there was a lack of toxicological response in these COC derived ingredients.

PMID:
21651430
DOI:
10.3109/08958378.2010.542580
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Taylor & Francis
    Loading ...
    Support Center